multispot5_headless command not found

In this troubleshooting guide we learn how to fix multispot5_headless command not found error message

Introduction

When you run more command in linux terminal / console, you get the following error message

multispot5_headless: command not found

or when using sudo you get the following error message

sudo: multispot5_headless: command not found

Solutions to multispot5_headless: command not found

How To Fix multispot5_headless: command not found in Ubuntu / Debian / Kali Linux / Raspbian

In Ubuntu multispot5_headless is provided by threeb package.

threeb is:

Bayesian analysis of blinking and bleaching, or 3B microscopy, is a method which analyses data in which many overlapping fluorophores undergo bleaching and blinking events, giving the structure at enhanced resolution. By using a Hidden Markov Model (HMM), it allows useful information to be obtained from data that would be impossible to analyse with standard localisation analysis techniques.

This package contains the command-line tool.

To fix this problem, we can install more using the command below.

sudo apt-get -y install threeb

This command might take some time to finish depending on your machine internet connection.

You can also use apt command to install threeb.

sudo apt -y install threeb

Or if you have aptitude installed you can use the following command.

sudo aptitude install threeb

Summary

In this tutorial we learn how to fix multispot5_headless command not found error in Ubuntu / Debian / Kali Linux or Raspbian distribution.